2孔腔室載玻片-無(wú)凹液面-80296 80292 80293 80294
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貨號(hào)
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產(chǎn)品名稱(chēng)
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規(guī)格(個(gè)/盒)
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80296
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µ-Slide 2孔Ph+腔室載玻片,ibiTreat底部處理
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15
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80292
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µ-Slide 2孔Ph+腔室載玻片,Collagen IV底部處理
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15
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80293
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µ-Slide 2孔Ph+腔室載玻片,F(xiàn)ibronectin底部處理
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15
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80294
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µ-Slide 2孔Ph+腔室載玻片,Poly-L-Lysine底部處理
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15
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80295
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µ-Slide 2孔Ph+腔室載玻片,Poly-D-Lysine底部處理
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15
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80291
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µ-Slide 2孔Ph+腔室載玻片,無(wú)包被
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15
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80297
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µ-Slide 2孔Ph+腔室載玻片,玻璃底
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15
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μ培養(yǎng)載玻片 2孔Ph+
一個(gè)開(kāi)放的兩孔培養(yǎng)載玻片,并帶有一個(gè)特殊中間板,非常適用于相差和高端的熒光顯微鏡
整個(gè)培養(yǎng)孔具有優(yōu)秀的相差-沒(méi)有彎月面現(xiàn)象
一體化小室,既可用于細(xì)胞培養(yǎng),也可用于顯微鏡成像
使用高分辨率顯微鏡可以通過(guò)玻璃片底部進(jìn)行樣品觀察
沒(méi)有蓋玻片,不會(huì)泄露,用于快速,簡(jiǎn)單的免疫熒光。
應(yīng)用:
細(xì)胞培養(yǎng)以及細(xì)胞培養(yǎng)物的顯微鏡觀察。
轉(zhuǎn)染分析
活細(xì)胞和固定細(xì)胞的免疫熒光顯微鏡觀察
長(zhǎng)時(shí)間段下活細(xì)胞成像

規(guī)格:
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孔數(shù)量
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2
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帶蓋子高度
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10.8 mm
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孔尺寸大小
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2.2 x 23.3 x 9.3
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每孔生成面積
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4.8 cm²
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每孔體積
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1500 µl
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每孔包被面積
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11.4 cm²
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底部 ibidi 標(biāo)準(zhǔn)底部
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技術(shù)特點(diǎn):
開(kāi)放式載玻片,具有兩個(gè)獨(dú)立的培養(yǎng)孔
每個(gè)培養(yǎng)孔上的中間板可以避免彎液面形成
從兩側(cè)縫隙很容易加液,無(wú)氣泡形成,
適用于高端顯微鏡的優(yōu)秀光學(xué)成像特質(zhì)
兼容染色,固定
采用最佳細(xì)胞粘附的表面處理Ibitreat
生物兼容性塑料生產(chǎn),無(wú)膠水,不泄露
相差顯微鏡是最常用的,細(xì)胞培養(yǎng)中的透射光學(xué)技術(shù)。使用相差顯微鏡時(shí),把兩個(gè)相位環(huán)彼此調(diào)節(jié)好是非常重要的。
在開(kāi)放式培養(yǎng)孔中,空氣-水交界面處的彎月牙就像一個(gè)棱鏡折射出光路。這個(gè)會(huì)誤校相位環(huán),造成顯微鏡圖像的對(duì)
比性較差。使用µ- 2孔ph+培養(yǎng)載玻片減少了彎月牙,無(wú)論孔在哪個(gè)位置,整個(gè)光學(xué)系統(tǒng)都是平行的。
µ-Slide 2 well µ-Slide 2 well Ph+


The µ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when
normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 well, the Ph+ version provides a
special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast
effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of
liquids.This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Cross Section Through a Well of the µ-Slide 2 well Ph+ with a Transmitted Light Beam Path

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading
to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.Working with the µ-Slide 2
well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the
parallel beam path that is created by the plate.